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Mouse G-CSF MAb (Clone 67604R)（貨號 MAB414R）

G-CSF is a pleiotropic cytokine known for its specific effects on the proliferation, differentiation and activation of neutrophil lineage hematopoietic cells. It is mainly produced by activation of endotoxin, TNF-α and IFN-γ by monocytes and macrophages, or by LPS, IL-1 or by fibroblasts, endothelial cells, astrocytes and bone marrow stromal cells. TNF-α is secreted after activation.

Human G-CSF has 73% homology with murine G-CSF at the amino acid level, and both proteins have species cross-reactivity. In vitro, G-CSF stimulates cell growth, differentiation and function of the neutrophil lineage. Consistent with its in vitro function, G-CSF has been found to play an important role in protecting against infection, inflammation, and maintaining steady-state hematopoietic capacity. Recombinant human G-CSF has been approved to improve chemotherapy-induced neutropenia and severe chronic neutropenia after bone marrow transplantation.

The use of recombinant monoclonal antibodies can reduce the variables in the experiment and improve the repeatability of the experiment. R&D Systems' recombinant monoclonal antibodies include rabbit, rat, mouse and other species, undergoing rigorous sequencing validation and batch-to-batch testing to ensure extremely high batch-to-batch consistency.

Mouse G-CSF monoclonal antibody (product no. MAB414R) is a recombinant monoclonal antibody that can be used in a ELISA assay: the antibody is coated as a capture antibody in a polystyrene microplate, added to the sample to be tested, and then used Goat anti-mouse G-CSF monoclonal antibody (Catalog #AF-414-NA) was used as a detection antibody, labeled with biotin, and HRP-labeled streptavidin was added after incubation with proteins on microplates, followed by color development. The substrate is finally added to the stop solution to terminate the reaction.

This product is recommended for use in immunoassay experiments where antibody pairs are required. For a simple ELISA assay, use the Mouse G-CSF DuoSet ELISA Kit (Catalog # DY414), DIY your own ELISA kit, or use the complete Mouse G-CSF Quantikine ELISA Kit (Catalog # MCS00) for peace of mind.

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